terça-feira, 26 de junho de 2012

Seminário em Microbiologia Agrícola - ALINE SUELEN DA SILVA - LIGNIN DEGRADATION, LIGNINOLYTIC ENZYMES ACTIVITIES AND EXOPOLYSACCHARIDE PRODUCTION BY GRIFOLA FRONDOSA STRAINS CULTIVATED ON OAK SAWDUST (1-2012)

LIGNIN DEGRADATION, LIGNINOLYTIC ENZYMES ACTIVITIES AND EXOPOLYSACCHARIDE PRODUCTION BY GRIFOLA FRONDOSA STRAINS CULTIVATED ON OAK SAWDUST

Nona A. Mikiashvili; Omoanghe S. Isikhuemhen*; Elijah I. Ohimain

Mushroom Biology & Fungal Biotechnology Laboratory, School of Agriculture and Environmental Sciences, North Carolina A&T State University, Greensboro, NC 2741, USA.

ABSTRACT
Fourteen strains of Grifola frondosa (Dicks.) S. F. Gray, originating from different regions (Asia, Europe
and North America) were tested for lignin degradation, ligninolytic enzyme activities, protein accumulation
and exopolysaccharide production during 55 days of cultivation on oak sawdust. Lignin degradation varied
from 2.6 to7.1 % of dry weight of the oak sawdust substrate among tested strains. The loss of dry matter in
all screened fungi varied between 11.7 and 33.0%, and the amount of crude protein in the dry substrate
varied between 0.94 to 2.55%. The strain, MBFBL 596, had the highest laccase activity (703.3 U/l), and the
maximum peroxidase activity of 22.6 U/l was shown by the strain MBFBL 684. Several tested strains
(MBFBL 21, 638

Key words: Grifola frondosa; exopolysaccharide; laccase; lignin degradation; peroxidase

Brazilian Journal of Microbiology (2011) 42: 1101-1108


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